FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965587
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1148181.0470757288232724No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC375820.3427267505150431Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41050.048.42844418
CACACGT39200.041.60707519
AGAGCAC72450.039.66159415
GAGCACA71350.037.96753316
AGCACAC60450.037.63417417
CAGATCG95850.030.417136
GATCGGA334700.029.0288018
AGATCGG338050.028.6790127
TAGATCG91850.028.4647126
GGTCGCC193950.028.18750250
CGCCGTA182950.028.06499953
TCGGAAG348050.027.98574810
ACACGTC26450.027.52358620
TCGCCGT185700.027.47963752
GTCGCCG195500.027.26581851
ATCGGAA355950.027.1679739
GCCGTAT191000.027.08372754
GTGGTCG200450.027.01155748
TGGTCGC197950.026.87530749
CCGTATC183850.026.67115655
Produced by FastQC (version 0.11.5)