FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16473691
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1803101.0945330952243792No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC198980.12078653168861792Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG69250.048.92391618
CACACGT68450.041.67252719
AGAGCAC107700.041.0118515
AGCACAC94900.039.7574217
GAGCACA107650.039.2101916
ACACGTC47050.030.20174820
GATCGGA264000.028.0131038
AGATCGG266500.027.8422497
TCGGAAG274750.026.49667210
ATCGGAA289000.025.2628369
TAGATCG81700.024.6326686
AAGAGCA189900.024.05200214
CAGATCG93800.023.9924076
CGGAAGA304950.023.79229411
GAAGAGC323600.022.41026113
CGCCGTA99550.021.48154847
GGTCGCC103650.020.76689144
GTCGCCG105650.020.34063545
TCGCCGT102500.020.0779446
CTCGGTG99800.019.8846638
Produced by FastQC (version 0.11.5)