HNC3GAFXY_n02_ncm3-15.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HNC3GAFXY_n02_ncm3-15.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5268379
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	953387	18.096401189056444	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	74096	1.4064288085576229	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	25820	0.4900938220276104	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	16328	0.309924551745423	Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	15145	0.2874698270568613	Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC	13541	0.25702402959240406	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	13032	0.2473626138134709	Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC	13004	0.24683114103977713	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	12603	0.2392196916736628	Illumina Single End PCR Primer 1 (100% over 41bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC	12235	0.23223462093368757	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	10568	0.2005930097284193	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC	10184	0.19330424026061907	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC	9985	0.1895269873332955	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	9157	0.17381057816835122	Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCC	8228	0.15617707078401155	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC	7427	0.14097315322227197	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC	6627	0.12578821683102145	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCTCGGTGGTCGCC	6304	0.11965729876305406	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	6201	0.11770223820268055	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC	5958	0.11308981377383821	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	5878	0.11157132013471316	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG	5672	0.10766119901396616	Illumina Single End PCR Primer 1 (100% over 42bp)
AGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGATCTCGGTGGTCGCC	5636	0.10697787687635987	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	5566	0.10564919494212546	Illumina Single End PCR Primer 1 (100% over 41bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGCA	40	1.3460522E-10	70.01499	1
GCGTCCC	15	0.002225385	69.9997	18
ACACTCT	12000	0.0	69.19815	1
CGCGCCA	365	0.0	69.05588	1
GCCCCAG	835	0.0	68.74288	2
CACTCTT	11305	0.0	68.57622	2
GTGACTG	7650	0.0	68.55062	1
GCCGCAG	920	0.0	68.47862	2
CGGCCCA	430	0.0	68.386734	1
GGCCGCA	400	0.0	68.26462	1
GCCTAAG	190	0.0	68.15826	2
CCGGGCA	345	0.0	67.98557	1
CCGCAAG	325	0.0	67.84651	2
GCCCGCA	315	0.0	67.79229	1
GGACCAG	630	0.0	67.77814	2
GCCCCAA	155	0.0	67.75645	1
GGCTTAG	460	0.0	67.71775	2
CCGACAG	295	0.0	67.62748	2
ACGGGCA	290	0.0	67.60068	1
GGCCAAG	705	0.0	67.51809	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content