HNC3GAFXY_n02_ncm3-14.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HNC3GAFXY_n02_ncm3-14.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6352400
Sequences flagged as poor quality	0
Sequence length	76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	981343	15.448381713997858	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	89693	1.4119545368679554	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	22388	0.35243372583590454	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	19366	0.30486115483911597	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	16721	0.26322334865562624	Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	15573	0.24515143882627038	Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC	14143	0.22264026194824005	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	13480	0.21220326175933504	Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	13230	0.20826774132611295	Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	12811	0.20167180908003277	Illumina Single End PCR Primer 1 (100% over 41bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC	12493	0.19666582708897426	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC	12208	0.19217933379510108	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC	11041	0.17380832441282035	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC	10916	0.1718405641962093	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTTGGGAAAGAGTGTAGATCTCGGTGGTCGCC	9603	0.1511712108809269	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC	8915	0.1403406586486997	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCTCGGTGGTCGCC	8898	0.1400730432592406	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC	8747	0.13769598891757445	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATGTCGGTGGTCGCC	8461	0.13319375354196838	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	8310	0.13081669920030223	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT	8001	0.12595239594483976	Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGCGGTGGTCGCC	7810	0.12294565833385807	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCC	7452	0.11730999307348405	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCC	6838	0.10764435488949059	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC	6493	0.10221333669164409	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGCA	150	0.0	70.016624	1
CCCCGCA	185	0.0	70.016624	1
AGCCGCC	30	1.0250915E-7	69.99954	21
GCCTTCG	15	0.0022254267	69.99954	23
TTACGCC	15	0.0022254267	69.99954	44
TGCCTAG	345	0.0	68.985596	2
ACACTCT	13215	0.0	68.77153	1
CACTCTT	12485	0.0	68.430214	2
CCCGCAG	615	0.0	68.29278	2
GTGACTG	8140	0.0	68.2103	1
CCGCTAG	480	0.0	67.81259	2
CCAGGGT	685	0.0	67.44481	26
CCCCCCA	135	0.0	67.42342	1
CGGGCCA	495	0.0	67.187675	1
CGCCAAG	710	0.0	67.04234	2
CAGGGCA	320	0.0	66.7346	1
CCCGGCA	300	0.0	66.51579	1
CGGCCCA	465	0.0	66.2523	1
GCCCCAG	1105	0.0	66.19919	2
CGCGCAA	275	0.0	66.19754	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content