FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6179746
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC89073014.413699203818409Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG764531.2371544073170644No Hit
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC272800.4414420916328924Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC257110.4160526986060592Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT255920.4141270531183644Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG171220.2770664037000874Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC160010.2589264995681052Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC133660.21628720662629175Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC130900.2118210036464282Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC118870.19235418413637065Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC102620.16605860499768113Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC99140.16042730558828794Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC95450.1544561863869486Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG82860.13408318076503467Illumina Single End PCR Primer 1 (100% over 41bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCC78560.12712496597756606Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC78560.12712496597756606Illumina Single End PCR Primer 1 (100% over 49bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC71300.11537691031314232Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC68950.11157416502231646Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT68250.11044143238249599Illumina Single End PCR Primer 1 (100% over 43bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC61950.10024683862411173Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT180550.069.103141
CACTCTT172300.068.559132
TAACTGA88100.068.5314562
CGGGCCA4300.067.5720751
GTGACTG103750.067.4837951
GCGCGCA5500.067.468461
ATAACTG89150.067.4620361
CGCGGCA5100.067.268771
CCCGCGA1200.067.097171
GCGGACA2300.066.970331
GGCCCCA4950.066.4783551
GCGGCCA4900.066.442271
ACGGCCA1900.066.329461
CGGTCCA1900.066.329461
GCGGTCA2800.066.2636641
CAGGCCA2800.066.2636641
CGCGCCA4400.066.036351
GCCGGCA3450.065.955631
CGGGCTA2550.065.895941
GCAGCCA1700.065.895941
Produced by FastQC (version 0.11.5)