FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3763231
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72760.19334449572720888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC28900.035.9686435
TCGCGAG3300.033.939642
CGCGAGA5700.031.9296323
AAAAGGG122050.031.85970170
GCGAGAT13300.029.9998174
CATTAAA264000.029.24603561
CCGTATC266800.029.04482355
CGTATCA268950.028.77359456
CTAGATC24600.028.7396645
GTATCAT254300.028.42173857
TCATTAA260050.028.3451360
CAAGATC39300.028.3204385
CGCCGTA287200.028.2728153
TCGCCGT290150.027.85266552
GCCGTAT293900.027.83146554
ATCATTA273300.026.86846259
GTCGCCG316600.026.59806851
CGCGTAG3050.026.3936332
CGCGAAA5450.026.3360631
GGTCGCC322200.026.32044850
Produced by FastQC (version 0.11.5)