FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985163
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22680.23021571049663864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC6200.036.1287465
CGCGAAT952.8140857E-729.4854221
TCGCGTA755.5721324E-528.011151
GCCCGTC500.007210158727.99977720
CGCGTAG1207.397284E-826.253792
CGCGCAG1101.0131334E-625.4646821
AAAGGGG18150.025.26150770
CCGTATC39150.024.94232755
CAAGATC7600.024.8682235
CGCGGAT851.3053016E-424.715721
CGCCGTA42450.024.24008853
TGCGGTA1601.8553692E-924.072081
CCGAGAT1752.0190782E-1023.999814
CGTATCA41300.023.89811556
GCCGTAT46550.023.53364854
TCGCCGT44300.023.46482552
CATTAAA42100.023.36085961
CGGACTG750.00182860523.3426271
CGCGAAG1052.0339703E-523.3426251
TGTCGTG4200.023.3426251
Produced by FastQC (version 0.11.5)