FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840990
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35710.1939717217366743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC12200.041.8851665
TCGCGAG1850.035.9458772
CGCGAGA2700.034.999933
GCGAGAT5450.034.6788334
CGCGTAG1450.031.379252
CCGAGAT3750.030.799944
CGCGAAG2650.029.0604931
CATTAAA114050.027.74216561
TGCGTCG4050.027.6542662
AAAAGGG52300.027.37088470
TCATTAA111850.027.16132460
CGGCAAG3750.027.0702921
CAAGATC17800.026.741525
CCGTATC118750.026.67363255
TCGAGAT5650.026.6371154
GTATCAT111100.026.58860657
TGTCGTG7250.026.5552791
CGTATCA120000.026.42494856
CTCGCGA1207.405288E-826.2535131
CTAGATC12150.025.9258755
Produced by FastQC (version 0.11.5)