FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988113
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1620342.3187089275745825TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226050.3234778830851762No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG129730.18564382115744263TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG117930.1687580037701165TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCA76080.10887059210404869TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG108300.043.76476370
GTGACTG45200.039.8658451
TGACTGG47100.038.6192972
GGATCTC20600.035.3598139
TGATCGG30250.034.9862751
GACTGGA52700.034.580663
CCCGATC18850.034.56799737
ACTGGAG53700.034.323084
CGTGGAA37300.031.88885961
TGCGTGG23200.031.66309759
GCTTGAA219150.031.49470160
CTGCTTG204100.031.16049458
CTTGAAA232750.031.11756361
ACGTGGG47950.030.360887
TGCTTGA209800.030.1486759
CACTTAC29250.029.40067732
AAAAAGG226500.029.26507470
TCACATA74900.029.10714731
GCGTGGA39100.028.96932460
GAGTTGG33950.028.8242658
Produced by FastQC (version 0.11.5)