FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720517
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT18750.050.5980071
GCGTGAA13250.036.7166866
GCTTGAA96650.035.48858366
GGCGTGA5750.034.08667465
CTTGAAA101150.034.08276467
GAAAAAA124950.033.89327270
TGCTTGA92500.033.67539665
CGTGAAA14900.033.59032467
TGAAAAA122500.033.37114769
CTGCGTG9200.033.09755364
CTGCTTG93950.033.04389664
CGGCGTG5900.032.62684664
TTGAAAA110950.031.7347568
GCGAGAT6750.031.1126634
TGCGTGA9800.031.07117365
CGGCTTG10800.030.7867864
CACTCTT43400.030.3293781
TCTGCTT104600.029.9471763
CGCGAGA3100.029.3563023
TCGGCTT11000.029.27248463
Produced by FastQC (version 0.11.5)