FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779175
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT46500.047.6592751
CACTCTT121900.029.4098551
GGGTGGA25700.029.14375566
CGGCTTG13600.028.82331758
GCGTGGA9200.026.63023866
CCGGCGT12250.026.00008257
TGCCGGC14300.025.94413855
CGGCGTG20450.025.50103658
GCGTGGG19200.025.15606560
GCTTGGG9300.024.8385360
CGTGGGG17150.024.69369761
GCGTGAA10350.024.68581266
GTGGAAA40050.024.64401468
GGGGGAA33950.024.53590267
GGCGTGA6300.024.44426565
GCGGCGT15950.024.13800857
GGGGGGA36600.024.09818566
TGGCGGC20750.023.27718255
GGAAAAA94400.022.8017470
GCCGGCG10750.022.7907756
Produced by FastQC (version 0.11.5)