FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10271571
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT60200.034.6609541
TGCCGGC24600.024.32915555
GCGTGGG24350.024.14761460
GCCCACA27300.023.9811341
GGGTGGA28600.022.63964766
CGGCGTG26850.022.29039858
GCGTGAA23850.022.15911766
GCGGCGT21800.021.03201157
CCGGCGT19450.020.33409757
CACTCTT143900.020.2663231
GGGCGTG19350.020.25820564
GGGGGGA36400.020.09596366
ATCGCGT46400.019.91370247
GTGGGCG22050.019.52362362
GGCGTGA15450.019.48201465
GCCGGCG19750.018.96193756
GCCGGCT16150.018.854456
CGTGGGG22450.018.70806161
GGGGGAA37650.018.68508167
CGGCTTG20000.018.54991358
Produced by FastQC (version 0.11.5)