FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7405342
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT93540.1263142201940167TruSeq Adapter, Index 6 (100% over 39bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA82030.11077138638566592TruSeq Adapter, Index 6 (100% over 40bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA81360.10986663411358989TruSeq Adapter, Index 6 (100% over 38bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA75630.10212897662255167TruSeq Adapter, Index 6 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACG27000.058.2031412
ACACTCT137150.058.0205461
AGGTCAC29100.054.36373511
TCACGAA37850.041.70369314
GGCGTGA109750.040.30940665
CGTGAAA254700.039.32824767
ACGAAAG41100.039.2575316
GCGTGAA249950.039.1374566
GAAAAAA909550.038.96889570
GTGAAAA308850.037.91771768
GCCGGCT76300.037.84367456
CGGCTTC71850.037.5083458
CCGGCTT83600.037.42786857
CACGAAA43550.037.2901115
TGAAAAA856600.036.85465269
TCGGCGT108350.035.98488663
GCGTCGG40150.035.9149760
CGGCGTC56950.035.09184658
GCTTGAA481300.034.92694566
GGGCGTG60200.034.8833964
Produced by FastQC (version 0.11.5)