FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437738
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT32250.047.3338781
GCGTGAA23600.042.11822566
CGTGAAA28250.037.41555467
TGCGTGA13850.036.64223565
GCCGGCT13450.034.6093256
GGCGTGA13850.034.3678965
CTGCGTG13500.033.96262464
CCGGCTT17650.032.3226257
TGCCGGC28600.031.3283655
GTGAAAA50850.029.73421768
GGGCGTG14750.029.1861564
GGGTGGA17700.028.86976866
CGGCTTC15050.028.83692458
CACTCTT80450.028.4185851
TGGGCGT14550.028.38459463
GCGTGGA10950.028.12757366
CCGGCGT19500.027.99972257
GAAAAAA129350.027.8698470
CGGCGTC12850.027.23708258
GCCCACA12550.027.0609681
Produced by FastQC (version 0.11.5)