FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689923
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG467180.3681503819999538TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA43750.029.83975865
TGCCGGC74000.029.46644255
GCGTGAA92650.028.02999366
AAAAGGG166200.027.81866670
GCCGGCT39850.027.7542956
CTGCTTG103900.027.38679558
ATGCCGT137900.026.54811148
GCGTGGA62600.026.3895660
CATCTCG302600.026.13990840
GAGATCG153900.025.9718136
CCGGCTT55200.025.93316857
TCTCGTA210000.025.4664642
GTATGCC187850.025.45105246
CTCGTAT200950.025.4464243
CCGGCGT55150.025.38550657
TGCCGTC133100.025.29686449
ATGCCGG71200.025.16873254
TATGCCG165200.025.06345447
GCGTGGG47200.025.06335660
GCATCTC330450.024.9642439
Produced by FastQC (version 0.11.5)