FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8692216
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC36050.025.14540755
GGATCGC84700.025.08242245
ATGCCGT49700.024.71809248
GCGTGAA40650.024.28022666
GATCGCG77850.023.87262246
GCCGGCT21300.023.16880856
ATCGCGT50950.022.9438647
GATCTCG78250.022.58765840
GGCGTGA20350.022.53051465
TATGCCG56650.022.42698547
TGCCGTC50050.022.37742449
GAGATCG71450.022.1421816
GCGTGGG25650.021.83216560
CCGGCTT30500.021.80308557
TGGATCG170300.021.57937244
GGATCTC84600.021.55418239
GTATGCC66650.021.21511746
GCGTGGA29450.021.03546760
ATGCCGG36350.020.99018554
AAGATCG91000.020.9238556
Produced by FastQC (version 0.11.5)