FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965587
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG385390.3514540534856912TruSeq Adapter, Index 22 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA20050.024.6132165
TATGCCG232400.024.4275753
GCCGTCT187600.024.32821556
GCGTGAA46950.024.07855666
TGCCGTC219650.024.02899755
GTATGCC244350.023.3904952
ATGCCGT227850.023.28711154
CGTATGC245800.023.2382751
CGTACGA249950.023.03436741
GATCTCG203150.023.01734246
CCGTACG261250.022.96243940
CGATCTC205800.022.82289145
TACGATC220350.022.53890843
CTCGTAT222650.022.49481849
GAGATCG69650.022.463416
CCGTCTT206350.022.37205157
CGGCGTG20150.022.23301964
GTACGAT249450.022.05629542
TCGTATG247650.022.01884850
AGATCGG322750.022.0038727
Produced by FastQC (version 0.11.5)