FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16473691
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG154700.014.68312438
AGTCACG161650.014.57147135
ACGTGGC155050.014.55968639
CGTGGCC156850.014.34797240
GTCACGT167750.014.10419236
CGGCGTG19400.014.07203658
TCACGTG166900.013.81952537
GCACACG202150.013.71246318
CACACGT204000.013.57095319
GATCGGA206250.013.5077558
ACACGTC204500.013.50354420
AGATCGG210250.013.201157
ACGTCTG209450.013.167722
CACGTCT211850.013.08460921
GTGGCCA168350.012.97285441
CAGTCAC186150.012.86047834
CGTCTGA211600.012.83542223
GAACTCC207400.012.8253422
AGCACAC217800.012.759294517
GAGCACA222250.012.55106516
Produced by FastQC (version 0.11.5)