FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5268379
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG91394317.34770790028584TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATT311790.5918139146784998TruSeq Adapter, Index 4 (97% over 48bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG253050.48031851922574287TruSeq Adapter, Index 4 (100% over 49bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA247010.46885389225034874TruSeq Adapter, Index 4 (100% over 39bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC241220.4578637945371812TruSeq Adapter, Index 4 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG223940.4250643319320801TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTCTCGTATG201700.3828502087644036TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTG198270.37633966728665497TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATG196530.373036943621558TruSeq Adapter, Index 4 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA183100.34754523165474616TruSeq Adapter, Index 4 (100% over 39bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA170270.32319238991727817TruSeq Adapter, Index 4 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATG168990.3207628000946781TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATG155400.29496738940004125TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATG148790.2824208357067705TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAAG133310.25303798378970077TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATG120000.22777404586875774TruSeq Adapter, Index 4 (97% over 49bp)
CAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC110450.2096470280517024TruSeq Adapter, Index 4 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC78800.14957162345381758TruSeq Adapter, Index 4 (100% over 50bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC78230.14848969673594098TruSeq Adapter, Index 4 (100% over 37bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA76040.14433282039883616TruSeq Adapter, Index 4 (100% over 40bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT73400.13932179138972348TruSeq Adapter, Index 4 (97% over 48bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT70340.13351355322007016TruSeq Adapter, Index 4 (100% over 41bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG68410.129850187315681TruSeq Adapter, Index 4 (97% over 49bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC65820.1249340641590136TruSeq Adapter, Index 4 (100% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC63080.11973322344501032TruSeq Adapter, Index 4 (100% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC58020.11012875117754437TruSeq Adapter, Index 4 (100% over 46bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG55320.10500383514549731TruSeq Adapter, Index 4 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC150.002225327870.0001655
AGAATCG13350.066.604551
GAATCGG14100.065.0380862
AGATTCG4200.062.512011
AATAGGG9700.060.61812270
ATAAGGG4300.058.60423370
AAAAGGG484100.058.27989670
TGATCGG58250.058.2343641
AAATGGG17450.057.56405670
AAACGGG16550.056.67632770
GATTCGG4850.056.2909242
AGATGGA12400.056.1801451
AAAGGGG80400.056.0692570
AGAAGGG7250.055.99959670
ACAGGGC252.391912E-455.99959670
TTGGAAA72450.055.89331462
AGACGGA9100.055.7799451
ATTCGGA4950.055.1532143
AACAGGG7600.054.34171370
GCTTGAC1550.054.19315760
Produced by FastQC (version 0.11.5)