FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6352400
Sequences flagged as poor quality0
Sequence length76
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1340912.1108714816447325TruSeq Adapter, Index 3 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG594700.9361816006548707TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATT460310.724623764246584TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATG430440.6776021661104464TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATG358020.563598010200869TruSeq Adapter, Index 3 (97% over 49bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC306000.4817077010263837TruSeq Adapter, Index 3 (100% over 39bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG295080.46451734777406967TruSeq Adapter, Index 3 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTG286460.4509476733203199TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATG273210.4300894150242428TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTT270500.42582331087463005TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTG240910.3792424910270134TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATT235620.37091492979031543TruSeq Adapter, Index 3 (97% over 48bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA217810.34287828222404126TruSeq Adapter, Index 3 (100% over 40bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC212070.33384232730936336TruSeq Adapter, Index 3 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATG204920.3225867388703482TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATT199150.31350355771047167TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATG195870.3083401549020843TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATT195650.3079938291039607TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTG188050.29602984698696555TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTG171290.26964611800264465TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTG167650.26391600025187334TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTT167480.2636483848624142TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATT157420.24781185063912853TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTT138450.2179491215918393TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATT135030.21256532963919147TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTG131320.2067250173162899TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATT130940.20612681821044015TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTT124790.19644543794471384TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATG119560.18821232919841321TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTT114840.1807820666204899TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATG111670.1757918267111643TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTG106760.1680624645803161TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTT105030.16533908444052642TruSeq Adapter, Index 3 (97% over 46bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA98370.15485485800642276TruSeq Adapter, Index 3 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTG97830.1540047855928468TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTG93110.1465745230149235TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATG90890.14307978087022227TruSeq Adapter, Index 3 (97% over 41bp)
CAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC89730.14125369938920723TruSeq Adapter, Index 3 (100% over 42bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG84760.13342988476796172TruSeq Adapter, Index 3 (100% over 37bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT82830.13039166299351426TruSeq Adapter, Index 3 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTT82270.1295101064164725TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATT82160.12933694351741074TruSeq Adapter, Index 3 (97% over 45bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG81280.12795164032491657TruSeq Adapter, Index 3 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGTATG81240.127888671997985TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATT78800.12404760405516026TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGATG74450.11719979850135381TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGTTG72810.11461809709716012TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTG72730.11449216044329702TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTT68910.10847868522133366TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGTTT68210.10737673950003147TruSeq Adapter, Index 3 (97% over 45bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG66180.10418109690825514TruSeq Adapter, Index 3 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAAG64630.10174107423965746TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATT63680.10024557647503306TruSeq Adapter, Index 3 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATCG14400.065.399731
AAAAGGG419600.062.24190570
AAAGGGG45500.058.23010670
TGATCGG87450.057.7687151
AAATGGG15300.056.73138470
AAACGGG9100.056.5378270
AATAGGG7650.053.52880570
AGATTCG4450.053.4977041
CTGCTTG213150.052.6931758
TGCTTGT44250.052.5259
CTTGTAA62650.052.45750461
GAATCGG18900.052.4101142
GCTTGTA53950.052.35343660
TTCTGCT159250.052.1980356
AGATGGA16800.051.889121
CTTGGAA66500.051.3678461
AACAGGG4650.051.18221370
TGCTTGA198450.050.89969359
CAATCTC4950.050.2014539
AGACGGA11950.050.097311
Produced by FastQC (version 0.11.5)