FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6179746
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG92034314.892893656147033TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA650101.0519849844961264TruSeq Adapter, Index 14 (97% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG422800.6841705144515648TruSeq Adapter, Index 1 (100% over 39bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC418690.6775197556663332TruSeq Adapter, Index 1 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATG342040.5534855316059916TruSeq Adapter, Index 1 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG316200.5116715153017616TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATG286510.46362746947851907TruSeq Adapter, Index 1 (97% over 49bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA251820.40749247622798734TruSeq Adapter, Index 1 (100% over 40bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG230690.3733001324002637TruSeq Adapter, Index 1 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATG203770.3297384714517393TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG148220.23984804553455757TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATG141990.22976672504015538TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATT132440.21431301545403325TruSeq Adapter, Index 1 (97% over 48bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA123780.20029949450996853TruSeq Adapter, Index 1 (100% over 37bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA122980.19900494292160228TruSeq Adapter, Index 1 (100% over 40bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT103380.16728842900662907TruSeq Adapter, Index 1 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAAG94380.15272472363750872TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCGATCTCGTATG93270.15092853330865053TruSeq Adapter, Index 1 (97% over 49bp)
CAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC79810.12914770283438834TruSeq Adapter, Index 1 (100% over 42bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT79200.12816060724825906TruSeq Adapter, Index 1 (100% over 41bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG78920.12770751419233087TruSeq Adapter, Index 1 (100% over 49bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG78700.12735151250553017TruSeq Adapter, Index 1 (97% over 49bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA73260.11854856170463964TruSeq Adapter, Index 1 (100% over 40bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT72110.11668764379636316TruSeq Adapter, Index 1 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC70920.11476199830866836TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATG66030.10684905172477963TruSeq Adapter, Index 1 (97% over 49bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT65610.10616941214088733TruSeq Adapter, Index 1 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGGG9300.059.8381470
GAATCGG13400.059.817222
AGAATCG12900.059.433911
AAACGGG15100.058.4100470
TGATCGG69700.058.3651051
AGATTCG3550.058.1841
TGTAGGG7900.056.280321
AAAAGGG508000.056.17860470
AAATGGG20000.055.64947570
ATAAGGG4450.055.05565670
TTGGAAA57050.053.4964262
AAAGGGG96650.053.3414570
GATCCGG5400.052.5033262
AGAAGGG9150.052.40387370
GTGGAAA103600.051.48662
AACAGGG8300.051.44529770
GTAGGGA8450.051.36422
AAGAGGG32500.050.9379870
GATTCGG4400.050.1168142
GGATCGG24300.049.704161
Produced by FastQC (version 0.11.5)