FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3763231
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37850.048.006191
GCGTGAA47650.042.2345666
GGCGTGA22500.040.2884265
CGTGAAA53850.039.9716867
GCGTGGG19400.036.62328760
TGCGTGA28050.036.5592965
GCCGGCT21350.036.06563656
GTGAAAA83550.035.9421368
GCGTCGG11400.035.92063560
GGGCGTG20250.035.43168664
CGGCTTC21800.034.8395158
TCGGCGT22050.034.761563
TGCCGGC41150.034.61731355
CGTGGGC13150.034.6003661
CCGGCTT27250.034.55051857
GAAAAAA200250.034.2567870
CCGGCGT25450.034.2436757
TGGGCGT20750.033.9032263
CGGCGTC17050.033.8710358
CTGCGTG28750.033.8430964
Produced by FastQC (version 0.11.5)