FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985163
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT12500.054.058931
GCCCACA3050.044.7697751
GCGTGAA12050.036.01641566
CGTGAAA12650.035.4148467
CGGGCTG806.249866E-834.9998226
CACTCTT31500.034.7899631
GGCGTGA6250.033.59982765
GAAAAAA49700.030.35196170
GCTTGAA26250.030.26651466
GTGAAAA17250.030.23173368
TGCGTGA7200.030.13873365
GTCGGGG1052.0259904E-829.9998562
GTACGCG703.4904646E-529.99984721
CTGCGTG7350.029.04747264
TGAAAAA49150.028.9113569
GCGTGGA2450.028.57128366
GGGGTGA1850.028.37823565
CTTGAAA29250.028.3588367
AGGCCGC1004.423764E-727.99985916
TACGCGC755.5874654E-527.99985722
Produced by FastQC (version 0.11.5)