FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n01_ncm3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n01_ncm3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840990
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT26050.052.5406041
GCGTGAA8200.042.25599366
GGCGTGA4500.037.3332565
CGGCTTG8050.036.52165258
GGGTGGA11850.035.44295566
TGCCGGC11900.035.29403755
CGGGCTG3400.034.9999226
CCGGCTT8300.034.99991657
CGTGAAA11150.034.52906467
GCGTCGG4800.034.27075260
CGGCGTC5950.034.1175758
GGCCGCC3500.033.99992417
GGGCTGC3550.033.5210527
CCGGGCT3450.033.47818425
CCGGCGT8850.033.22026457
GCGTGGG11400.033.15781860
CGTGGGC4950.032.52517761
GCTTGGG5000.032.19992460
GCCGGCT6200.032.17734556
GCGGCGT9800.032.1427857