FastQCFastQC Report
Tue 24 May 2016
HNC35BGXX_n01_nlp6_pn_pchx_pd_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC35BGXX_n01_nlp6_pn_pchx_pd_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6855631
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC258280.37674139696258446No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG228210.33287964302629475No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT215090.31374209026127575No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG171870.2506990239118762No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA168850.24629388600407462No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC107900.157388867633045No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC101450.1479805432935349No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG101040.14738249476962806No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG98740.1440275884160043No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC97160.14172291361655842No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC96290.1404538838219268No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA94690.13812003592375377No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT94550.13791582423266363No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC91050.1328105319554101No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT90780.13241669512259338No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA87250.1272676431972491No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC79010.1152483265216579No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT76920.11219973770466933No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG76530.11163086227948966No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT75050.10947205297367958No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG74470.10862603311059187No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA72390.10559203084296691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT47050.033.122981
CTAGCAG53350.029.5214528
CTGACTC57250.027.3889142
ACTCTAG57700.027.113865
GACTCTA59750.026.476284
CTCTAGC64450.024.5456856
TAGCAGA65500.024.2054629
TGACTCT65750.024.0069663
CGGGGGG5650.022.3142871
CTATCGA73450.021.20464317
TCTATCG77250.020.11611416
GATCTAT78150.019.92894614
CCCTACA6350.019.841710
ATCTATC78000.019.78778815
AGCAGAT83900.018.89671710
AGATCTA84650.018.60539413
GCAGATC85700.018.49995411
TAGCATG86200.017.86547527
CTAGCAT86750.017.7926826
GCATGAC87750.017.6695629
Produced by FastQC (version 0.11.2)