FastQCFastQC Report
Tue 24 May 2016
HNC35BGXX_n01_nlp6_pn_pchx_md_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC35BGXX_n01_nlp6_pn_pchx_md_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8470309
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC265660.3136367280107491No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG245110.2893755115663431No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT240810.2842989553273676No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG180890.21355773443448167No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA167690.19797388737530117No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC119500.1410810396645506No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC110710.13070361423650542No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC109210.1289327225252349No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG106080.12523746182105044No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC104030.12281724314898075No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT103500.12219152807766517No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT103170.12180193190118566No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC102660.1211998287193537No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG100280.11839001387080447No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA97220.11477739477981264No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA92800.10955916720393553No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA86620.10226309335350105No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT85200.10058664920016495No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT84960.10030330652636167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT59800.030.5662041
CTAGCAG64650.028.1505228
ACTCTAG69200.026.5529375
GACTCTA71050.026.2063734
CTGACTC70050.026.1308122
CTCTAGC76500.024.3393826
TAGCAGA75100.024.3266459
TGACTCT81300.023.0313843
CTATCGA85800.021.3344517
TCTATCG92950.019.69322216
ATCTATC95300.019.28071815
GATCTAT95050.019.18414314
AGCAGAT99850.018.64726810
TAGCATG98950.018.32342527
CTAGCAT99950.018.1050826
AGATCTA103550.017.84609413
GCAGATC103900.017.65092311
GCATGAC106150.017.04699529
TCTAGCA156600.016.8735667
TTCTAGC107700.016.83425724
Produced by FastQC (version 0.11.2)