FastQCFastQC Report
Tue 24 May 2016
HNC35BGXX_n01_nlp6_pn_pchx_md_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC35BGXX_n01_nlp6_pn_pchx_md_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10447674
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC221570.21207591278211785No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT172560.16516594985639865No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG164790.1577288877888035No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG160460.15358442462886954No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG127890.12241002159906597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT35400.036.112621
CTAGCAG39250.032.6361548
CCCTACA7050.031.27569210
GACTCTA43300.030.6345084
ACTCTAG43150.030.4978165
CTCTAGC46850.027.6408736
TGACTCT48900.027.054823
TAGCAGA47800.026.7251669
CTATCGA51250.024.92610717
GCGGGGG9800.024.3025741
CTGACTC53950.023.6789862
GATCTAT56200.022.97987214
ATCTATC56350.022.67004215
TCTATCG56350.022.60804216
AGCAGAT59250.021.91497610
TCCCTAC13950.021.8273249
AGATCTA60450.021.36404213
TAGCATG62150.020.72444727
TATCGAT62800.020.62032318
CTAGCAT62650.020.61511226
Produced by FastQC (version 0.11.2)