FastQCFastQC Report
Tue 24 May 2016
HNC35BGXX_n01_nlp6_pn_mchx_md_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC35BGXX_n01_nlp6_pn_mchx_md_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7912203
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC370540.4683145768631063No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG317980.4018855431287595No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT316120.3995347439897586No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG274120.34645218278651346No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA234490.2963649946797371No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG143740.1816687463655824No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC139200.17593077427361256No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG137350.1735926138396601No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC136190.17212652405404666No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC135050.17068571167853No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA134740.17029391182202985No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC134730.17028127311698146No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT133930.1692701767131101No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC129450.16360803685143063No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT128540.162457914692027No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA121550.1536234598632012No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA116530.14727882992890856No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC109650.13858340085561507No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT107830.13628315653680775No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA104700.13232724185666117No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT101920.12881368185320827No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT100890.1275118952332239No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG100730.12730967595244966No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG91000.11501221594036452No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG90090.11386209378096088No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTG88460.11180198485807302No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCAT88030.11125852054099218No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAG87690.11082880456934688No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC87650.1107782497491533No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG85180.10765648960220055No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT83850.10597554183076445No Hit
CCTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGC81130.10253781405760191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT67250.030.8288211
CTGACTC69700.030.2804372
ACTCTAG70950.029.5490725
GACTCTA73000.029.0069394
CTAGCAG75350.027.5897588
CTCTAGC81750.025.6881566
TGACTCT84850.024.7907283
TAGCAGA84800.024.5151929
CTATCGA102550.020.30684717
TCTATCG105700.019.60233916
ATCTATC107100.019.44401615
GATCTAT107950.019.35563314
AGATCTA107400.019.29169513
AGCAGAT110850.018.78575910
TATCGAT113900.018.46789618
GCAGATC115150.018.11464311
CCGGGGT5700.017.2032571
TGCGCAT112700.017.17505369
GTGCGCA112600.017.09683268
TCTAGCA185500.016.7349517
Produced by FastQC (version 0.11.2)