FastQCFastQC Report
Tue 24 May 2016
HNC35BGXX_n01_nlp6_pn_mchx_md_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC35BGXX_n01_nlp6_pn_mchx_md_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7125949
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC455450.6391429408209349No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG342710.48093243440277217No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG326670.4584231517795034No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT298080.41830217982194373No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA142350.19976286667221446No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA138550.19443024360685152No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA138470.19431797785810703No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC138340.19413554601639726No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG135230.18977121503395547No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT129430.18163194824998044No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC126790.17792717854141252No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAG123420.17319798387555116No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC122000.1712052668353366No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG121970.17116316717955743No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC115760.16244853843326693No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA113100.15871570228751286No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT112880.15840697147846552No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG112690.1581403403251974No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG111210.15606342397342446No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC109440.15357954428245277No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCAT106650.1496642762949889No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT105710.14834515374724125No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACC103510.14525784565676797No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC102810.14427552035525373No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC101760.1428020324029824No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT101140.14193197285021264No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC99500.13963052500095074No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC99070.13902709660144916No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA98260.13789040589541127No Hit
GCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCC97500.1368238812823387No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG93630.13139302568682432No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC93090.13063523188279905No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC92280.12949854117676116No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT91700.12868461449836366No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG91540.1284600830008747No Hit
GGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT90960.1276461563224772No Hit
GCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAG90860.1275058241365466No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCC90450.1269304621742311No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT87740.12312745993551175No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAG85790.12039098230986497No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC83010.11648974754099418No Hit
GTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCG79840.11204121724699406No Hit
GTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCA79720.11187281862387731No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT77940.10937490571431258No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG77050.1081259492595302No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG76210.10694715889771314No Hit
TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC76030.10669456096303803No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT75850.10644196302836295No Hit
GCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC75300.10567013600574465No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG74810.10498250829468468No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT74770.10492637542031244No Hit
GGGGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCA74610.10470184392282347No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTG74260.10421068127206637No Hit
GCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTAC74130.10402824943035657No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGC73680.10339675459366887No Hit
GTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCT72930.10234426319918934No Hit
CTGGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG72190.10130580502330286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT59650.037.807281
CTGACTC60900.037.128812
ACTCTAG64250.035.4643065
GACTCTA66500.034.3694084
CTAGCAG66250.034.1276058
CTCTAGC73500.031.048736
TAGCAGA75200.030.3451299
TGACTCT77500.029.6718223
CGTATGC9100.027.69155146
GTATGCC9100.027.30694847
CGTCCGC9800.026.42784733
CCGCACA9600.026.24965136
TATGCCG9700.025.9786748
CGCACAT10000.024.84966937
AGATCTA93200.024.33392713
CTATCGA93550.024.13099117
AGCAGAT94000.024.12716910
TCTATCG93500.024.1064616
GATCTAT94500.023.88823314
CCGGGGT5000.023.8127161
Produced by FastQC (version 0.11.2)