FastQCFastQC Report
Tue 24 May 2016
HNC35BGXX_n01_nlp6_pn_mchx_md_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC35BGXX_n01_nlp6_pn_mchx_md_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9180507
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC517570.5637706065688964No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT396700.43211121128713265No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG390770.4256518730392559No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG328520.3578451604034505No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA169310.1844233657247906No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC169160.18425997605578864No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC168950.1840312305191859No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC154200.1679645797339951No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT153110.166777281472581No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT152210.16579694345856935No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC150480.16391251594274694No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG148130.16135274446171655No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA146250.15930492727689222No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG139520.15197417746100514No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG139030.15144043787559883No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC137030.14926190895557293No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA136810.14902227077437008No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAG135880.14800925482655805No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT133630.14555840979152893No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT131920.14369576756490682No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG131660.14341255880530346No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA130190.14181134004908444No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC125640.13685518675602557No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC123870.13492718866180267No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT122800.13376167568958883No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC121400.13223670544557073No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACC117650.12815196372052218No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA115030.12529809083528828No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC114900.1251564864554866No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC114880.12513470116628636No Hit
GTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCG114190.12438310868887743No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCAT112780.12284724580025917No Hit
GCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCC106980.11652951193218415No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC106840.11637701490778232No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG104880.11424205656615698No Hit
GGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT104790.1141440227647558No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG104390.11370831698075062No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAG102970.11216156144753225No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT101600.11066926913731452No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCC94310.10272853122382021No Hit
TCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC94120.10252157097641774No Hit
GTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCA92920.10121445362440222No Hit
GCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAG92440.10069160668359602No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC92160.10038661263479239No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT91910.10011429651978916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT80650.037.1701431
ACTCTAG85650.035.0209165
CTGACTC86650.034.5363432
GACTCTA90550.033.086434
CTAGCAG92350.032.782458
CTCTAGC97500.031.1953056
TGACTCT100950.029.7124943
TAGCAGA104050.028.894229
CTATCGA122500.024.4570817
AGCAGAT125650.024.03854410
AGATCTA126350.023.98833813
TCTATCG125350.023.87309516
GATCTAT127550.023.59814514
ATCTATC128700.023.46925215
CGCGGGG4550.023.0905741
TATCGAT133900.022.55782118
GCGGGGG14250.022.3640981
GCAGATC135750.022.22437911
ATTCTAG145800.020.62054823
CTAGCAT144450.020.61965226
Produced by FastQC (version 0.11.2)