FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_nlp7_4tu_pdex_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_nlp7_4tu_pdex_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17903924
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG340410.19013150413283703No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT301630.16847144793510072No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT296870.16581281287833885No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG273610.1528212474539101No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA229060.12793843405501498No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG218860.12224135893338242No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC207250.11575674695670066No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT205400.11472345391993397No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA202270.11297523380908008No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC191220.10680340242731146No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA190910.10663025602655597No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG190810.10657440234889291No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT184520.10306120602388616No Hit
GCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTA180710.10093318090492341No Hit
GCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACG180480.10080471744629838No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC180050.1005645466323472No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG179240.10011213184327637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA13700.042.14294410
CTACACG13900.037.0040712
GTATGCC8850.035.9877147
ACACTCT16300.035.6494671
TATGCCG9250.034.81092548
CGTATGC9150.034.80895646
TCGTATG9750.032.6643145
TCCCTAC17100.032.5357869
CTCGTAT10350.030.43199244
CCGGCAT10650.029.5818581
TACACGA17750.028.97718813
ACACGAC17550.028.7092214
GCCGGAT14450.026.1630551
CGCGGTT16450.025.7485051
GACGCTC15300.022.86931218
ATGCCGT14750.022.78069149
CCCGCTT9100.022.6956791
CTAGCAG10950.022.3691928
CTGCTTG14850.021.69169459
GCGGGCC9050.020.5002821
Produced by FastQC (version 0.11.2)