FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_nlp7_4tu_pdex_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_nlp7_4tu_pdex_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20860163
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG419270.20099075927642562No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT374610.1795815305949431No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG338670.1623525185301764No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT317430.1521704312665246No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG267140.12806227832447906No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT252080.12084277577313274No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT251500.12056473384220438No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA248890.11931354515302686No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA248440.11909782296523762No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG247280.11854173910338094No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA229770.11014774908518213No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC226890.10876712708333103No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC219850.10539227330102839No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG216920.10398768216720071No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTT214440.10279881322116229No Hit
GCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACG213280.10224272935930559No Hit
GGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAG211370.10132710851780016No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC210380.10085251970466387No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT209500.10043066298187603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA12300.040.1121410
CCGGCAT10350.038.5560841
GTATGCC8400.035.83135645
ACGGATA39600.034.819816
CTACACG12850.033.76565612
TATGCCG9250.032.9188446
AACGGAT43100.031.9916765
CAACGGA43600.031.8653684
CGTATGC9500.031.6819344
ACACTCT16700.031.4414961
TCGTATG9750.031.22822243
GGCAGAC74800.030.5590611
CGCGGTT17000.030.0630021
CGCGGGG9100.029.6195031
GCCGGAT15050.029.538991
CTAGCAG13800.029.1599528
CTCGTAT10650.027.93145842
CCAGGCA29800.027.0101852
TACACGA17100.026.80547313
ACACGAC16650.026.689414
Produced by FastQC (version 0.11.2)