FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_nlp7_4tu_pdex_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_nlp7_4tu_pdex_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19943821
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT477900.23962308927662357No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG422110.2116495128992584No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT374600.18782759833233562No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA304730.15279419124349342No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA300540.15069328991671155No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG295210.14802078297834703No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA277490.13913582557725523No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT271090.13592681161749295No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG269680.13521982572948282No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG254000.12735774152806525No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA242920.12180213611022682No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC230930.11579024901998468No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG223710.11217008014662788No Hit
GCCGGCGATGCGTCCTGGTCGGATGCGGAACGGAGCAATCCGGTCCGCCG223510.1120697984603853No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTT222900.11176393931734546No Hit
GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA217610.10911148871622946No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC215080.10784292538526091No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC215050.10782788313232455No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT212540.10656934796998029No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG210670.10563171420361224No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA209290.1049397705685385No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCG204880.10272855938688981No Hit
CGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAG204000.10228731996742249No Hit
CGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTG201800.1011842214187542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA10450.041.1857610
CCGGCAT11750.038.729351
CGTATGC6900.038.54977446
TATGCCG7450.035.70471648
CTACACG11100.034.99001312
GTATGCC7750.034.77316747
TCGTATG8450.032.3055245
CGCGGGG7850.032.106841
ACACTCT13550.032.034441
CTAGCAG13350.029.8803148
TCCCTAC14250.028.484179
CGTATCT9700.028.14229639
CTCGTAT9600.027.70633144
GCCGGAT12550.027.61381
ACACGAC14100.027.54519314
CCCCCTT13100.027.2560921
TACACGA15250.025.9268313
GGCAGAC27950.025.549541
CGCGGTT15650.024.6044161
CCCGCTT7850.023.6342011
Produced by FastQC (version 0.11.2)