FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_nlp7_4tu_mdex_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_nlp7_4tu_mdex_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20686262
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG482620.23330459606476997No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT403570.19509082887957235No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG386080.18663594224998215No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG369980.1788529991547047No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT309580.14965487723204898No Hit
CGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTG301110.1455603723862726No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA298060.1440859639117014No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT289520.13995762018290206No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG287940.13919382825181273No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT280990.13583411058024886No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT272430.13169609859915726No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA266780.12896481732659093No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC249860.12078547588733045No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG249540.12063078385065411No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC242500.11722755904377505No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA236930.11453495078037781No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG234500.113360258126867No Hit
GCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACG234260.11324423909935975No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAA231290.11180850363395765No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC225610.10906271998295293No Hit
GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTA223260.1079267003386112No Hit
GTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCC222590.10760281388682016No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT221730.10718707903825254No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTT218020.10539361823803645No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA216100.10446546601797851No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA211500.10224176799075638No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC210770.10188887678208851No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC210600.10180669663760424No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC210420.10171968236697378No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG209080.1010719094633917No Hit
GCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACGTGAG208030.1005643262180475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA11100.047.91805310
GTATGCC7950.041.38132547
TATGCCG8150.040.366948
CTACACG10800.040.1750612
CCGGCAT9050.039.0676461
ACGGATA47250.038.886896
ACACTCT13500.038.3771171
CGTATGC8700.038.21725546
CAACGGA50400.036.802864
TCCCTAC13900.036.755199
AACGGAT51200.036.2282565
TCGTATG9450.035.92321445
ACACGAC12600.034.43534514
TGCGGGG3650.033.5675471
GCCGGAT11650.032.452061
GGCAGAC96900.032.29671
GCGGTAT5000.032.2056661
CTGCTTG11000.031.82870559
CTCGTAT10450.031.48072244
TACACGA14800.030.73512313
Produced by FastQC (version 0.11.2)