FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_nlp7_4tu_mdex_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_nlp7_4tu_mdex_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21254202
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG452430.21286614289259131No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT376010.1769108997834875No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG338960.15947905265979875No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT326610.1536684369519025No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG281730.1325526124198876No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG280930.13217621626067164No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA270230.12714191763115829No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT253670.1193505171353881No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA249460.11736973234751415No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC238070.11201079203067704No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC231870.10909372179675342No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTT230840.10860911174176288No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA228450.10748462821610523No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAA227440.10700942806509507No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT227160.1068776894093695No Hit
CGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTG219170.10311843276920019No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG218450.10277967622590582No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC218300.10270910194605284No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC218280.10269969204207242No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA217450.10230918102688588No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC216060.10165519270024816No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG215890.10157520851641479No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA214710.10102002418157126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA13200.044.8000910
TATGCCG9200.043.36969448
CTACACG12300.041.53379412
GTATGCC9600.040.83268747
CGTATGC10000.039.1998446
ACACTCT15850.036.6619531
CCGGCAT10750.036.1452561
TCGTATG10950.035.4769745
ACACGAC15800.033.8828614
ACGGATA35200.032.610286
CTCGTAT12700.030.58833944
TACACGA17650.030.52971313
GGCAGAC66600.030.4853081
CGCGGGG7950.029.5015241
GCCGGAT11850.029.2451341
CAACGGA39750.029.1410394
TCCCTAC20000.029.0435249
AACGGAT39700.029.0015755
CCCGCTT8300.027.4139791
CTGCTTG14700.027.39141359
Produced by FastQC (version 0.11.2)