FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_nlp7_4tu_mdex_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_nlp7_4tu_mdex_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23495999
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT431010.18343974223015588No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG385460.16405346288957537No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT363710.15479656770499522No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG335250.14268386715542505No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG279470.11894365504526963No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC272130.11581971892320903No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA264310.11249149270052318No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT242970.10340909531022709No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA242750.10331546234744052No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC241840.10292816236500521No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA237440.10105550310927405No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG235200.10010214930635637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA15050.044.6404910
GTATGCC11350.040.08688747
TATGCCG12350.036.5591548
CGTATGC12550.035.97584546
CCGGCAT11300.035.0058671
TCGTATG13050.034.595445
CTACACG17500.034.19044512
TCCCTAC19550.034.007519
CTCGTAT13650.032.81804744
ACACTCT21200.029.7219641
CTAGCAG14600.028.5209488
ACACGAC20700.027.89028414
GCCGGAT17350.027.6415211
CGCGGGG8750.027.6046281
TACACGA21600.027.53807413
CGCGGTT19200.026.6190451
ATGCCGT18000.025.08406649
CTGCTTG18600.024.65960759
GGCGTAT8450.022.3706131
GCGGTAT7800.021.0932771
Produced by FastQC (version 0.11.2)