FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_cont_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_cont_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21214678
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG9150.011.8601411
CTCGAAT80950.09.7732911
CTGGGAT83550.09.7624471
CTCGGAT92500.09.7261421
CGGGAAT50800.09.7163841
CGGGCAT24150.09.5670051
GGGGACT43200.09.4809391
AGGGGGC18700.08.981338515
GGGGATT77100.08.8991991
CTGGAAT145600.08.8478081
CTCGTAT50850.08.6741881
CTCGCAT48200.08.6426941
CTGGCAT48600.08.5715611
CTCGGTT83350.08.3578911
CGCGTAT14900.08.2230141
GGGGAAT59600.08.1642781
CTCAAAT174150.08.1008541
CTCGGGT51050.08.091621
CGCGGGG5653.409104E-58.0546031
TAGTATG91050.07.994952734
Produced by FastQC (version 0.11.2)