FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_cont_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_cont_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18098409
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT18550.013.7758611
CGGGCCC3254.2381362E-712.9252131
CGGGGGG11200.010.6267561
CTGGGAT70900.010.0721861
GGGGAAT56650.09.8251021
CGGGGAT31150.09.4397621
CGGGAAT47300.09.3990161
CGGGGAG23200.09.3549941
CGGGGGT10500.09.3348761
CGGGGGA16600.09.2786431
GGGGACT33300.09.1456561
GGGGATT64550.09.0564941
CTCGAAT65850.08.9840211
CGGGGAC16500.08.9105631
CTGGAAT121450.08.7334321
CTCGGAT77300.08.6042671
CGCGCAC5351.7199265E-58.5060791
CTGGCAT39500.08.0646241
CGGGTAT29800.07.98789741
GGAAGAT228350.07.9868691
Produced by FastQC (version 0.11.2)