FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_cont_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_cont_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20160532
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG11150.016.6394081
CGGGGAG27050.012.0351781
CGGGCAT20750.011.3029961
CTCGGAT83700.010.455661
GGGGATT83900.010.222121
GGGGAAT66850.09.9492221
CGGGAAT47500.09.9489451
CGGGGAT35600.09.6363591
CTCGAAT72150.09.6065131
CGCCCAT26000.09.2899341
CTGGAAT136450.09.0816891
CCGGGAT44200.08.9493831
CTCGTAT49150.08.9027341
CTGGGAT79700.08.6964851
TAGTATG100200.08.66196334
GGGGACT48900.08.5903191
CGCGGGG5351.7201075E-58.50602151
GGGAGAT109800.08.4485161
CTCGGGT50100.08.38456251
GGTAGTA89500.08.25051429
Produced by FastQC (version 0.11.2)