FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_cont_p3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_cont_p3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19362576
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG12200.015.4944431
CGCGGGG8950.012.90722751
CGTAGCG28050.012.35057925
CGGGCAT20300.011.5536921
CTCGGCT69200.010.0667441
CGGGAAT51200.010.050541
CGGGGGA19450.09.718881
GCGGGGG13550.09.5588241
GGGGAAT70650.09.4142011
CCGGGGT14000.09.00153351
CTCGAAT71250.08.9418741
CCCGGAT31900.08.888661
CGCGGAT28150.08.8292131
CTGGGAT75800.08.8207641
TCGGCTC56350.08.818852
CCCGGGG13500.08.8163171
CCGGGAT53650.08.8085831
CTCGGAT78950.08.7348631
CGGGGGT14850.08.7220241
GGGGATT71550.08.7086811
Produced by FastQC (version 0.11.2)