FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_cont_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_cont_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23020883
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG17600.018.4964081
CGTAGCG39850.014.66457225
CGCGGGG10100.012.8232321
GCGGGGG16050.011.7770321
CTCGGCT87100.011.1723291
CGGGGAG34850.010.8477111
CGGGAAT60450.010.7704471
CGCATCG57950.010.62731410
TCCCGTG59300.010.56912661
CGGGCAT24250.010.3929261
CCCGTGA60350.010.32737862
CGGGGGT18350.010.3008911
ATCCCGT60800.010.13538860
CGGATAT70600.010.1144571
TCGGCTC73050.010.0602682
CGGGGAT46500.010.0118841
CCGGGAT60500.010.0093951
CGGGCCG5957.201379E-910.0011291
CGGGCGC10600.09.90271315
CCGGGGT16100.09.7837141
Produced by FastQC (version 0.11.2)