FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_cont_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_cont_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23904262
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG9650.014.5099481
CGTAGCG40800.013.72269625
CGGGGGG19050.012.8628231
CGGGCAT25450.011.141161
CTCGGCT90100.010.761881
GGGGACT40450.010.2116681
GCGGGGG20050.010.1262071
CCGGGGT16950.010.1195131
CGGGGGT19400.010.1046081
ATCCCGT58250.09.97791860
CGGGAAT63750.09.8289251
CCCGTGA59550.09.81919962
TCGGCTC77400.09.766232
CTCGCAT67300.09.5150718
CGCATCG64150.09.49124310
CTGGGAT85400.09.3456641
CTCGAAT88350.09.1920981
CTGGAAT152700.09.1238311
TCCCGTG64100.09.06750461
CCCGACT46500.09.0336131
Produced by FastQC (version 0.11.2)