FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_4tu_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_4tu_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18653816
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG558960.2996491441751114No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG423270.22690799566158476No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT411290.22048571723876767No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA405840.2175640630313926No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT402890.21598261717602446No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT401980.21549478133589395No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA387780.20788239789649474No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG368280.1974287727508409No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG312870.1677243948369599No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA291800.15642911884624572No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC282090.15122374960705093No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA277790.14891859124159904No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA274180.14698333038130107No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC230690.123669065889789No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA230120.1233634983855314No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA221790.11889792415664441No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC209160.11212719156230554No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT206860.1108941998784592No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA206340.11061543654124176No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC205680.11026162153631193No Hit
CAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTT201790.10817625734058918No Hit
CGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA191940.10289583643368198No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA191250.10252593892852808No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT190400.10207026808884574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19350.047.92938245
TATGCCG19500.047.20440746
CGTATGC20750.044.5268144
CCGGCAT10150.044.4879231
TCGTATG21650.042.67558343
ACGGATA54800.042.659926
CAACGGA55400.042.44914
AACGGAT56700.041.538015
CTCGTAT22850.039.9742942
CTGCTTG24850.037.75845357
CCAGGCA54000.037.652492
ATGCCGT25000.036.81864547
TGTAATC25700.035.54115336
GCCAGTA71000.033.7715261
TAATCTC26950.033.76345438
GCCGTCT28550.032.61114549
TGAACCA127450.032.58311566
CGCGGTT16250.032.526851
GTGAACC127950.032.3464165
CCCTACA7600.031.30918110
Produced by FastQC (version 0.11.2)