FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_4tu_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_4tu_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17375977
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG473770.27265804967398377No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA439720.2530620292602827No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT436040.25094416273686365No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA418940.24110298948945433No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG415330.23902540847055678No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT394390.22697428754653626No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG378790.21799637511030315No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT338380.19474012885721476No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG325720.18745420761088716No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC261280.15036852316275512No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA244690.14082085859114568No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC232580.13385146630891603No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA226910.1305883404426698No Hit
CAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTT226750.13049625928947764No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC226710.1304732390011796No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA226570.1303926679921365No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA221440.1274403160179137No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT216610.12466061620592615No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA203040.11685098340081827No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG200150.11518776757128535No Hit
CGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA199470.11479642267021876No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA196370.11301235032712119No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA190350.10954779693826712No Hit
CCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCAAAAACAGGGGTTGTA189450.10902984045156136No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC189080.10881690278480456No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC185610.10681989277495016No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT176550.10160579747544554No Hit
CGCGGATTACGGTGGCGGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAG175700.10111661634911234No Hit
GCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTT174880.10064470043900264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13000.046.03442845
TATGCCG13300.044.9981346
CGTATGC13600.044.00324644
ACGGATA43700.042.6050836
TCGTATG14250.042.48726743
CAACGGA45450.041.1944434
CATCTCG14950.040.26403439
AACGGAT46800.039.931945
CCGGCAT8650.038.040551
CTCGTAT16100.037.3871742
CCAGGCA42700.037.1269342
CTACATC16500.036.6937636
TGAACCA100700.036.02376666
GTGAACC101150.035.6563165
GCTACAT17000.035.2033135
CCCGCTT6750.034.7460981
CTGCTTG17600.034.41515757
CGCGGTT14400.033.546841
GGCTACA18300.032.8939634
TACATCT18600.032.3627637
Produced by FastQC (version 0.11.2)