FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_4tu_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_4tu_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26322677
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT578150.21963951462839437No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA529110.2010091906685631No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT525040.1994629953480795No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA521880.19826250954642646No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG520630.1977876338337472No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG469140.17822655347706468No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC319440.12135543812660088No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA312110.1185707669474499No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA298060.11323316393693544No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC297060.11285326336679206No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA292820.11124248494938414No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA290920.11052067386611171No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA282730.10740928819663745No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT278580.10583270083054243No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA273930.10406616317937571No Hit
CGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA267880.10176776473000827No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC265260.10077242523623262No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG264170.10035833361477635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC12150.037.15702445
CCGGCAT14650.032.258131
CGTATGC14300.031.32538444
TATGCCG14200.031.30203446
CGCGGGG7650.031.1164821
ACGGATA38800.029.3145526
CCCGCTT13100.028.0582791
TCGTATG16900.027.54107343
CCAGGCA38650.026.6206532
GCCAGTA79750.026.5563071
GTGAACC77750.025.76000465
CAACGGA45350.025.5431774
GGCAGAC79950.025.1763271
CGCGGTT28750.024.7173081
TGAACCA82450.024.63131566
AACGGAT47000.024.4977115
TGTGTGC173400.023.953547
CTGACAT180050.023.9141521
GAATGGG177550.023.9089782
GTGTGCG174000.023.7303038
Produced by FastQC (version 0.11.2)