FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_4tu_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_4tu_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880703
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG478120.253232096283703No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA426450.22586553053665429No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA423040.2240594537184341No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT411850.2181327676199345No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG389190.20613109586014883No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT356570.18885419679553245No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG345010.1827315434176365No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG313440.16601076771346915No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA300130.15896124206815815No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC282840.14980374406609753No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC265640.14069391378064683No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCG258530.13692816416846343No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC258100.13670041841132718No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT246280.13044005829655814No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA232860.12333227210872393No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG223670.11846486860155579No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA223120.11817356588893964No Hit
CCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCAAAAACAGGGGTTGTA221350.11723610079561125No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA220740.11691301960525516No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT215990.1143972234508429No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC209520.11097044426788558No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA208740.1105573240572663No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC208180.11026072493169349No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT207840.11008064689116714No Hit
CAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTT207060.10966752668054786No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC204380.10824808800816367No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC202120.1070510986799591No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA197860.10479482676042307No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT195760.10368258003952502No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA195610.10360313384517514No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG191220.10127800855720256No Hit
GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA190110.1006901067190136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC9300.038.00802245
CGTATGC9350.037.80467244
TATGCCG9400.037.6065746
CCGGCAT8000.037.1948781
ACGGATA31950.036.366046
CCAGGCA39750.033.632182
GTGAACC84750.032.6797365
TGAACCA85650.032.49968366
TCGTATG11150.032.01521343
AACGGAT36650.031.7977775
CGCGGTT16800.031.6729491
CAACGGA37300.031.3373174
GCCAGTA48200.029.9229491
CGCGGGG6100.029.8419861
TGTTACG39850.029.5069853
GGATATC64150.028.6412162
CGGATAT65500.028.433121
CGTGAAC99000.027.94031364
TATCTCG64700.027.8566885
CAGGCAG49500.027.501623
Produced by FastQC (version 0.11.2)