FastQCFastQC Report
Sun 15 May 2016
HN7YMBGXX_n01_js_4tu_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7YMBGXX_n01_js_4tu_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24594058
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG833370.33885014014360704No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG591780.24061909588080177No Hit
AAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAG567140.23060041575896098No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA559930.22766881333694503No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA546880.222362653613324No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT521990.21224232292206516No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG491790.1999629341363674No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT490450.19941808708428677No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT472430.19209111404063534No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA393540.1600142603550825No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC357010.1451610791517203No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTC348760.1418066103609254No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG336420.13678913825445155No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC323960.13172287387465703No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC309050.12566043391456588No Hit
GGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTC303320.12333060286350468No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCG298240.12126506329293035No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA286920.11666232550968204No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA285530.11609714834371782No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT278960.11342577137941204No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA274130.11146188237825576No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC271710.11047790486628925No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT271150.11025020759079286No Hit
GGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTC269990.10977854894869321No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT269620.10962810610595454No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA269390.10953458758208995No Hit
CCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCAAAAACAGGGGTTGTA264440.10752190630761299No Hit
CAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTT264060.1073673974420976No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC261380.10627770333793635No Hit
CTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTA253520.10308180943543355No Hit
CGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA251200.10213849215123426No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA249060.10126836327701592No Hit
GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGG247900.10079670463491629No Hit
CTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGC247740.10073164827048874No Hit
GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA246470.10021526337784517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT11350.041.945551
GTATGCC15350.039.4433445
ACGGATA62050.038.1274956
CCAGGCA81550.038.0647742
TATGCCG16500.036.90876446
CGTATGC16550.036.7944944
CGCGGTT19450.036.3558771
CAACGGA65400.036.3343474
AACGGAT66300.035.894135
TGAACCA167050.035.69463366
GTGAACC168150.035.33618565
TCGTATG18300.033.27569243
CGGATAT126000.032.2833371
CAGGCAG99250.031.9457043
CCCAGGC95450.031.6869641
CGTGAAC191900.031.10930664
GGATATC130900.030.8524572
TGTTACG46550.030.7481063
TATCTCG133100.030.290225
GATATCT138200.029.3487873
Produced by FastQC (version 0.11.2)