FastQCFastQC Report
Fri 17 Jun 2022
HN7V7AFX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7V7AFX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190931176
Sequences flagged as poor quality0
Sequence length10
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCATCGG17568461192.01462782589262No Hit
AGATCTCGGT52006042.7238108039516815Illumina Single End PCR Primer 1 (100% over 10bp)
ATACCATCGG22954961.2022635842351905No Hit
ATACCAACGG20492711.0733035028286841No Hit
AGACCAACGG9851850.5159895940723688No Hit
GGGGGGGGGG5297430.27745233182872137No Hit
ATACAATAGG3119800.16339919259702249No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)