FastQCFastQC Report
Wed 22 Jun 2022
HN7V3AFX3_n02_FMR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7V3AFX3_n02_FMR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43315974
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAAGCAACAAAAGTTATACGGTCTTTTCTCTCTATTAGATGATTA1128360.2604951235772743No Hit
CCTTGCGCAAATAGCGCTCCTCATCCGGCATGGCCATGGCGCCGCAGTAT666130.15378391352806703No Hit
CCTTGCGCACATAGCGCTCCTCATCCGGCATGGCCATGGCGCCGCAGTAT520470.12015659627092767No Hit
ATATTAACTCTTTTAAAATCGAGAAAAATAAATATTTATTTTTTATTTAA483650.11165626796248423No Hit
GGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACA442040.10205011204411564No Hit
ATATTATATTAACTCTTTTAAAATCGAGAAAAATAAATATTTATTTTTTA434430.10029325440078989No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)