FastQCFastQC Report
Wed 6 Jul 2022
HN7TMAFX3_n02_as4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7TMAFX3_n02_as4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7470772
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC157690.21107591022721614Illumina Single End PCR Primer 1 (100% over 50bp)
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG152270.20382097057707024Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT150850.20192022993072203Illumina Single End PCR Primer 1 (100% over 31bp)
TTTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG123050.16470854685432776Illumina Single End PCR Primer 1 (100% over 45bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG116560.15602135897066596Illumina Single End PCR Primer 1 (100% over 48bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG116100.1554056260852292Illumina Single End PCR Primer 1 (100% over 48bp)
TTTGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG115440.1545221832496026Illumina Single End PCR Primer 1 (100% over 45bp)
TGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG105870.14171226213301652Illumina Single End PCR Primer 1 (100% over 45bp)
TTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG105080.14065480782976644Illumina Single End PCR Primer 1 (100% over 45bp)
CAATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG101350.13566201725872507Illumina Single End PCR Primer 1 (100% over 45bp)
TGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG99910.1337345056173579Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98110.1313251160656489No Hit
CGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG97500.13050860071756976Illumina Single End PCR Primer 1 (100% over 32bp)
CGTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG95990.1284873905936361Illumina Single End PCR Primer 1 (100% over 45bp)
CGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT91130.12198203880402186Illumina Single End PCR Primer 1 (100% over 33bp)
TTTGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG89490.11978681721246479Illumina Single End PCR Primer 1 (100% over 44bp)
TTTGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG89410.11967973323238884Illumina Single End PCR Primer 1 (100% over 45bp)
TTTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG89280.1195057217647654Illumina Single End PCR Primer 1 (100% over 44bp)
TACAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG88790.11884983238680019Illumina Single End PCR Primer 1 (100% over 45bp)
CATAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG88020.11781914907856912Illumina Single End PCR Primer 1 (100% over 45bp)
CAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG86630.11595856492474942Illumina Single End PCR Primer 1 (100% over 45bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG86620.1159451794272399Illumina Single End PCR Primer 1 (100% over 48bp)
CATTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG85180.11401766778587274Illumina Single End PCR Primer 1 (100% over 44bp)
TTTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG84710.1133885494029265Illumina Single End PCR Primer 1 (100% over 44bp)
CACTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG84580.11321453793530308Illumina Single End PCR Primer 1 (100% over 45bp)
TTGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG83040.11115317131884093Illumina Single End PCR Primer 1 (100% over 45bp)
TGTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG82580.11053743843340422Illumina Single End PCR Primer 1 (100% over 45bp)
ACATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG82510.11044373995083775Illumina Single End PCR Primer 1 (100% over 45bp)
TTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG82020.10978785057287252Illumina Single End PCR Primer 1 (100% over 45bp)
TATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG81900.10962722460275857Illumina Single End PCR Primer 1 (100% over 45bp)
CATAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG80510.10776664044893887Illumina Single End PCR Primer 1 (100% over 44bp)
TTTTGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG79340.10620053724032805Illumina Single End PCR Primer 1 (100% over 44bp)
TGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG78920.10563834634492927Illumina Single End PCR Primer 1 (100% over 45bp)
TAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG78580.10518323942960647Illumina Single End PCR Primer 1 (100% over 45bp)
CAATAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG78110.10455412104666023Illumina Single End PCR Primer 1 (100% over 44bp)
TTTAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG77710.10401870114628047Illumina Single End PCR Primer 1 (100% over 45bp)
TAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG76930.1029746323405399Illumina Single End PCR Primer 1 (100% over 48bp)
TATTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG76500.10239905594763164Illumina Single End PCR Primer 1 (100% over 45bp)
TTTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG76460.10234551395759367Illumina Single End PCR Primer 1 (100% over 44bp)
CATTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG76410.1022785864700462Illumina Single End PCR Primer 1 (100% over 45bp)
CCATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG75710.1013416016443816Illumina Single End PCR Primer 1 (100% over 45bp)
CGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG75250.10072586875894485Illumina Single End PCR Primer 1 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)